goat anti mouse igs conjugated to allophycocyanin (SouthernBiotech)
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SouthernBiotech
goat anti mouse igs conjugated to allophycocyanin
Goat Anti Mouse Igs Conjugated To Allophycocyanin, supplied by SouthernBiotech, used in various techniques. Bioz Stars score: 93/100, based on 17 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/goat+anti+human+igm/Goat+Anti-Mouse+IgM%2C+Human+ads-APC/us12584929-471-37-47
Average 93 stars, based on 17 article reviews
Goat Anti Mouse Igs Conjugated To Allophycocyanin, supplied by SouthernBiotech, used in various techniques. Bioz Stars score: 93/100, based on 17 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/goat+anti+human+igm/Goat+Anti-Mouse+IgM%2C+Human+ads-APC/us12584929-471-37-47
Average 93 stars, based on 17 article reviews
goat anti mouse igs conjugated to allophycocyanin - by Bioz Stars,
2026-09
93/100 stars
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Clinical Proteomics:Article Title: A genome-wide CRISPR screen reveals novel determinants of long-lived plasma cell secretory capacity. Article Snippet: .. 215, No. 2 3 D ow nloaded from https://academ ic.oup.com /jim m unol/article/215/2/vkaf354/8502617 by guest on 09 M arch 2026 peroxidase-conjugated secondary reagents as appropriate: donkey anti-mouse IgG(HþL) for NP-specific plasma cells (Jackson Immunoresearch); goat anti-mouse IgM (1020-05), anti-IgG (1030-05), and anti-IgA (1040-05) for mouse splenic and bone marrow plasma cells; and Article Title: A genome wide CRISPR screen reveals novel determinants of long-lived plasma cell secretory capacity Article Snippet: .. Plates were washed the next day with 1xPBS and then incubated overnight with the following peroxidase- conjugated secondary reagents as appropriate: donkey anti-mouse IgG(H+L) for NP- specific plasma cells (Jackson Immunoresearch); goat anti-mouse IgM (1020-05), anti- IgG (1030-05), and anti-IgA (1040-05) for mouse splenic and bone marrow plasma cells; Incubation:Article Title: Distinct systemic and gut IgA responses to bacteria of the human upper gastrointestinal tract Article Snippet: .. Bound antibody was detected by incubation with AP-conjugated goat anti-human IgA (Sigma), goat anti-human IgG (SouthernBiotech) or Article Title: A genome wide CRISPR screen reveals novel determinants of long-lived plasma cell secretory capacity Article Snippet: .. Plates were washed the next day with 1xPBS and then incubated overnight with the following peroxidase- conjugated secondary reagents as appropriate: donkey anti-mouse IgG(H+L) for NP- specific plasma cells (Jackson Immunoresearch); goat anti-mouse IgM (1020-05), anti- IgG (1030-05), and anti-IgA (1040-05) for mouse splenic and bone marrow plasma cells; Recombinant:Article Title: Truncating RELA variants drive autoinflammation and autoimmunity by impairing the negative feedback control of NF-kB Article Snippet: .. In brief, 3.5 x 10 5 PBMCs were left untreated or stimulated with 15 μg/ml F(ab)’2 other:Article Title: Multiparametric Optimization of Human Primary B-Cell Cultures Using Design of Experiments. Article Snippet: ELISA was performed on dilutions of culture supernatant in duplicates using the following kits: Human BAFF/ BLyS/TNFSF13B DuoSet ELISA (#DY124- 05), Human IL- 4 DuoSet ELISA (#DY204- 05) and Human IL- 21 DuoSet ELISA (#DY8879- 05) (all from R&D Systems) according to the manufacturer's instructions. |
![( A to F ) Bead-based multiplex assays were used to measure plasma concentration of IL-6, and IL-12p70 (A); TNF-α, IFN-γ, and IL-1β [B; HC ( n = 19), irAE ( n = 34), RAC ( n = 45), and ICI ( n = 9)]; IP-10 (CXCL10), CXCL11, and CXCL9 (C; HC, n = 17; irAE, n = 33; RAC, n = 46; ICI, n = 17); CCL20 (D); CX3CL1 (E); and CCL2 (F). ( G to J ) Human naïve B cells were isolated and cultured <t>with</t> <t>anti–human</t> CD40 (0.5 μg/ml), anti–human Ig (M + G + A) (2.5 μg/ml), and rhIL-21 (20 ng/ml) with IFN-α (100 ng/ml), IL-6 (100 ng/ml), IL-12 (100 ng/ml), control, or the combination of IFN-α, IL-6, and IL-12 for 7 days. Cells and culture supernatants were analyzed. (G) Representative flow plot of CD38 and CD138 expression on CD27 hi CD38 hi ASCs. Right: A summary of the percentage of CD138 + ASCs; n = 6. (H) Expression of CD11c and CD27 on CD27 − IgD − B cells. Right: Percentage of CD11c + IgD − CD27 − B cells; n = 6. (I) Expression of active-caspase-3 in B cells. Right: Percentage of active-caspase-3 + B cells from different groups; n = 3. (J) Different immunoglobulin isotype levels in the culture supernatants from (G) to (H) were measured by the multiplex assay; n = 6. Data in graphs represent mean ± SEM. Significance was tested by one-way ANOVA [(A) to (I)] and paired Student’s t test (J). [(A) to (F)] ICI, ICI control.](https://pub-med-central-images-cdn.bioz.com/pub_med_central_ids_ending_with_1753/pmc13041753/pmc13041753__sciadv.aea4262-f5.jpg)
![( A to G ) CD8 + T cells isolated from healthy donor were cultured in the plate coated with anti–human CD3/CD28 (10 μg/ml) for indicated days in the presence of vehicle control (control), IFN-α (100 ng/ml), IL-6 (100 ng/ml), IL-12 (100 ng/ml), or the combination. (A) Summaries of the percentage of CD38 + CD127 − CD8 + T cells at days 1 and 5. (B) MFI of CD69 (day 5). (C) MFI of CD25 (day 5). (D) Activated CD8 + T cells were restimulated with PMA, ionomycin, and monensin for 5 hours; expression of granzyme B and IFN-γ in CD8 + T cells was examined. [(E) to (G)] CD8 + T cells were cultured for 5 days. MFIs (all relative to those under control condition) of MTDR (E), MTG (F), and GluCy5 (G) were summarized. ( H to L ) CD4 + T cells were isolated from HC, cultured in the plate coated with anti–human CD3/CD28 (10 μg/ml) for indicated days in the presence of IFN-α (100 ng/ml), IL-6 (100 ng/ml), IL-12 (100 ng/ml), control, or the combination of them. (H) Activated CD4 + T cells were restimulated with PMA, ionomycin, and monensin for 5 hours, and IL-21 level was examined in the CD45RA − CD4 + T cells. (I) Activated CD4 + T cells were restimulated with plate-coated anti–human CD3/CD28 (10 μg/ml) with monensin for 6 hours, and expression of CXCL13 was measured in CD45RA − CD4 + T cells. (J) CD38 and CXCR5 were examined on CD4 + T cells at day 5. (K) Percentage of CXCL13 + cells in indicated T cells at day 5. (L) Percentage of IL-21 + cells in indicated T cells at day 5. Data in graphs represent mean ± SEM. Significance was tested by one-way ANOVA.](https://pub-med-central-images-cdn.bioz.com/pub_med_central_ids_ending_with_1753/pmc13041753/pmc13041753__sciadv.aea4262-f6.jpg)
![( A to G ) PBMCs from the patients with irAE were cultured in the plate coated with anti-CD3 and anti-CD28 (10 μg/ml) in the presence of IgG1 isotype control or anti–human IL-6R (50 μg/ml), anti–human IL-12p40 (50 μg/ml), and anti–human IFNAR1 (50 μg/ml) for 3 days; n = 9. (A) Expression of CD38 and CD127 on CD8 + T cells. Right: Percentage of CD38 + CD127 − CD8 + T cells. (B and C) CD8 + T cells activated for 5 days were restimulated with PMA, ionomycin, and monensin for 5 hours. (B) Expression of granzyme B and IFN-γ. Right: Percentage of granzyme B + IFN-γ + CD8 + T cells. (C) Expression of perforin and IFN-γ. Right: Percentage of perforin + IFN-γ + CD8 + T cells. [(D) and (E)] MFIs of GluCy5 (D), TMRM, and MTDR (E) in CD8 + T cells were presented. [(F) and (G)] CD4 + T cells activated for 5 days were restimulated with PMA, ionomycin, and monensin for 5 hours. (F) Expression of IL-21 and IL-2. Right: Percentage of IL-21 + IL-2 + CD4 + T cells. (G) Expression of CD38 and CXCR5 on CD4 + T cells. Right: Percentage of CD38 + CXCR5 − CD4 + T cells. Data in graphs represent mean ± SEM. Significance was tested paired Student’s t test [(A) to (G)].](https://pub-med-central-images-cdn.bioz.com/pub_med_central_ids_ending_with_1753/pmc13041753/pmc13041753__sciadv.aea4262-f7.jpg)